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KMID : 1123920040180020553
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Korean Journal of Oriental Physiology and Pathology
2004 Volume.18 No. 2 p.553 ~ p.560
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Induction of Cdk Inhibitor p21 and Inhibition of hTERT Expression by the Aqueous Extract of Wikyung-tang in Human Lung Carcinoma Cells
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Choi Hae-Yun
Park Cheol
Choi Yung-Hyun
Park Dong-Il
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Abstract
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In the present study, we investigated the anti-proliferative effects of aqueous extract of Wikyung-tang(WKT) on the growth of human lung carcinoma cell line A549. WKT treatment declined the cell viability and proliferation of A549 cells in a concentration-dependent manner. The anti-proliferative effects by WKT treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. WKT treatment induced an inhibition and/or degradation of apoptotic target proteins such poly(ADP-ribose) polymerase (PARP) and phospholipase C-γ1 (PLC-γ1). WKT treatment did not affect the levels of other Bcl-2 family gene products, such as Bcl-2, Bax and Bad. Western blot analysis and RT-PCT data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 were induced by WKT treatment in A549 cells. Additionally, WKT treatment induced the down-regulation of telomerase reverse transcriptase mRNA (hTERT) expression of A549 cells, however, the levels of other telomere-regulatory gene products were not affected. Taken together, these findings suggest that WKT-induced inhibition of human lung cancer cell proliferation is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products and WKT may have therapeutic potential in human lung cancer.
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KEYWORD
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Wikyung-tang(葦莖湯), PARP, PLC-γ1, p53, p21, hTERT
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